Process Development for High Density Cultivation Yield for Bacillus Subtilis

Abstract

Bacillus subtilis is an aerobic, endospore forming, gram positive, and catalase positive bacterium. Bacillus subtilis is popular worldwide and have been extensively studied in medicine, biomaterials and agriculture sectors. In agriculture Bacillus subtilis is used on plants as a fungicide. This bacteria form rough biofilm which is beneficial for plant to allow for the control of plant pathogen infection. Bacillus subtilis are produce by fermentation technology. By conventional mode of fermentation process there is limitation to produce biomass. Currently, the demand of Bacillus subtilis is generated by its valuable biomass used in an agriculture sector. Therefore it is need to develop high cell density biomass to enhance production. The process development for Bacillus subtilis was carried in 10 L bench top lab scale glass fermenter with three different cultivation approaches. Batch fermentation process with maintained pH give up highest OD 30.4 mL-1 (against 21.85 in Shake flask and 26.2 in fermentation batch-1) with 100% sporulation efficiency.