Development and Validation of UV-Visible Spectrophotometric Method for Estimation of Apigenin in Parsley Leaves Extract

Abstract

A simple, precise and cost-effective UV- visible spectrophotometric method for the estimation of Apigenin according to the ICH Q2 (R1) guideline. The spiked Apigenin solution was scanned over UV-visible range for its wavelength of maximum absorbance. Various calibration standards of Apigenin were prepared and absorbance was recorded at wavelength of maximum absorbance. Calibration curve of concentration vs. absorbance was plotted and linearity and range was calculated. Various analytical method validation parameters viz. accuracy, precision, LOD, LOQ, robustness and ruggedness were calculated using QC standards. The maximum wavelength of Apigenin was found to be 268 nm. The correlation coefficient over the concentration range of 0.5-12 μg/mL was found to be 0.9997. Developed UV method was found to be precise during the intra-day and inter-day study and shows percent relative standard deviation in the range of 0.19 and 1.24 & 0.15 and 1.24respectively. The total percent recovery of Apigenin was found to be 99.94 and 100.10 %. Developed method was found to be robust and rugged for the intended use. Apigenin content of plant extract was successfully calculated using developed UV-Visible method. A simple, precise and cost-effective UV- visible spectrometry method for the estimation of Apigenin was developed. The said method was developed using economical percentage of organic phase in aqueous media as solvent. Said validated UV- visible method can be efficiently used for the estimation of Apigenin in bulk as well as in plant extract.