Determination of Humic Acid and Fulvic Acid by Spectrophotometric Analysis from the Effervescent Tablet

Abstract

This humic Acid and Fulvic Acid substances are applicable in agricultural, environmental, biomedicine. UV Spectrophotometric Analysis of the organic compounds like fulvic acid, humic acid. Humic acid (HA) represents the organic material most widespread in nature and have positive effects on plant physiology influencing nutrient uptake and root architecture. These are representing the most variations in chemical, functional groups and spectroscopic measurements were observed among the extracted humic and fulvic acids. More humic substances were extracted from the normal soil than other soil types, with the majority being humic acid. Fulvic acid extracted from all soils contained mostly aliphatic, O-substituted alkyl, and carboxylic groups and small amounts of carbonyl groups. Based on the spectroscopic analysis, no significant differences were detected among different types of humic substances. The aim of our work was to the application of UV-Vis spectrophotometry method to Humic acid determination after its extraction by Sodium pyrophosphate alkali solvent and fulvic acid extracted by layer separation technique. The analytical validation parameter of this method were evaluated. The results for the proposed method are comparable or more applied than them. Also this methodology is easier and faster than others methods.